Structural analysis of cell wall mannan of <Emphasis Type="Italic">Candida sojae</Emphasis>, a new yeast species isolated from defatted soybean flakes |
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Authors: | Hiroko Oyamada Yukiko Ogawa Nobuyuki Shibata Yoshio Okawa Shigeo Suzuki Hidemitsu Kobayashi |
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Institution: | (1) Department of Microbiology, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo Nagasaki, 859-3298, Japan;(2) Department of Infection and Host Defense, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai Miyagi, 981-8558, Japan;(3) Sendai Research Institute for Mycology, 1-14-34 Tohshogu, Aoba-ku, Sendai Miyagi, 981-0908, Japan |
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Abstract: | We investigated the structural and immunochemical characteristics of cell wall mannan obtained from Candida sojae JCM 1644, which is a new yeast species isolated from defatted soybean flakes. The results of a slide-agglutination test and
of an enzyme-linked immunosorbent assay using anti-factor sera to the pathogenic Candida species indicated that the cells and the C. sojae mannan were cross-reactive to the specific anti-factor sera against Candida albicans serotype A (FAb 6) and Candida guilliermondii (FAb 9). Two-dimensional homonuclear Hartmann–Hahn analysis indicated that the mannan consisted of various linked oligomannosyl
side chains containing α-1,2-, α-1,3-, α-1,6- and β-1,2-linked mannose residues. However, although the determinants of antigenic
factors 6 and 9 could be not found in this mannan, branched side chains, Manβ1-2Manα1-3Manα1-6]Manα1-(2Manα1-)n2Man and a
linear α-1,6-linked polymannosyl backbone, which are cross-reacted by FAbs 6 and 9, respectively, were identified. The mannan
was subjected to acetolysis in order to determine the polymerization length of the α-1,2-linked oligomannosyl residue in the
side chains. The result of 1H-nuclear magnetic resonance analysis of the released oligosaccharides showed that the remarkable regularity in the length
of α-1,2-linked oligomannosyl side chains, which were previously found in mannans of other Candida species, is not observed in this mannan. |
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Keywords: | Antigenic factor Candida sojae Cell wall mannan Nuclear magnetic resonance Polysaccharide antigen |
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