| 应用DGGE研究微生物群落时的常见问题分析 |
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| 引用本文： | 邢德峰,任南琪.应用DGGE研究微生物群落时的常见问题分析[J].微生物学报,2006,46(2):331-335. |
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| 作者姓名： | 邢德峰 任南琪 |
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| 作者单位： | 哈尔滨工业大学市政环境工程学院,哈尔滨,150090 |
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| 基金项目： | 科技部科研项目;中国科学院资助项目;国家杰出青年科学基金 |
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| 摘 要： | 变性梯度凝胶电泳(DGGE)是通过核酸片段对微生物群落进行研究,可以监测未培养细菌及其功能基因,被广泛地应用于微生物群落多样性和动态分析,并成为微生物分子生态学研究中的重要手段之一。文中论述了DGGE操作过程中遇到的常见问题,并提出了相应的解决方法。全面分析了样品预处理过程和PCR扩增效果对DGGE分析的影响,探讨了DGGE图谱的优化过程和图谱分析方法,并对DGGE的应用前景进行了综述。
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| 关 键 词： | DGGE 微生物群落 微生物分子生态学 图谱分析 排序 |
| 文章编号： | 0001-6209（2006）02-0331-05 |
| 收稿时间： | 2005-10-25 |
| 修稿时间： | 2005-12-13 |
Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis (DGGE) |
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| XING De-feng,REN Nan-qi.Common problems in the analyses of microbial community by denaturing gradient gel electrophoresis (DGGE)[J].Acta Microbiologica Sinica,2006,46(2):331-335. |
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| Authors: | XING De-feng REN Nan-qi |
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| Institution: | School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China |
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| Abstract: | Denaturing gradient gel electrophoresis(DGGE) can describe microbial communities based on partial sequences of nucleic acid and monitor uncultured bacterium and it's functional genes.It is well-established molecular tools in microbial molecular ecology that allows the study of diversity and dynamics of microbial communities.Here,common issues in the analyses of microbial community by DGGE were raised,and the feasible resolving methods were introduced in this paper.The effects of samples pretreatment and PCR amplification on DGGE analysis were evaluated.Optimization and statistical analysis of DGGE profiles were discussed.Finally,prospect of DGGE for understanding microbial communities were reviewed. |
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| Keywords: | DGGE Microbial community Microbial molecular ecology Profile analysis Ordination |
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