Demonstration of stage-specific nucleolytic activity in cell-free extracts ofPhycomyces and inhibition of this activity by EDTA and G-actin |
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| Authors: | Joe Fukui Kwan-Sam Choi Atsushi Miyazaki Tamotsu Ootaki Taneaki Oikawa |
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| Institution: | (1) Institute of Genetic Ecology, Tohoku University, Katahira 2-1-1, Aoba-ku, 980-8577 Sendai, Japan;(2) Kanto-Nousan Co. Ltd., Takaku-Kou-Michinishi 2691-3, Nasu Town, Nasu-gun, 325-0001 Tochigi, Japan;(3) Devision of Applied Biology & Chemistry, Chungnam National University, Gungdong, Yuseong-gu, 305-764 Deajeon, Korea;(4) Research Institute for Creative Biotechnology, Kasuminome 2-24-3, Wakabayashi-ku, 984-0035 Sendai, Japan |
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| Abstract: | Stage-specific nucleolytic activity was identified in cell-free extracts (CFEs) ofPhycomyces. Such activity was not detected in spore germlings or mycelia for the first 36 h after the start of cultivation. However,
it was detected in mycelia more than 48 h after the start of cultivation, as well as sporangiophores and sporangia. The nucleolytic
activity was completely inhibited by the addition of EDTA or G-actin to the CFE, and the various results together suggest
that the activity was due to deoxyribonuclease I (DNase I). |
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| Keywords: | DNase I EDTA G-actin Phycomyces stage-specific nucleolytic activity |
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