Synthesis of RNA oligomer using 9-fluorenylmethoxycarbonyl (Fmoc) group for 5'-hydroxyl protection |
| |
| Authors: | T Fukuda T Hamana R Marumoto |
| |
| Institution: | Biotechnology Laboratories, Central Research Division, Takeda Chemical Industries Ltd, Osaka, Japan. |
| |
| Abstract: | An approach using a new combination of protecting groups in RNA oligomer synthesis is proposed, in which 5'-hydroxyl group of ribose moiety is temporarily protected with the alkaline labile 9-fluorenylmethoxycarbonyl (Fmoc) group and the 2'-hydroxyl group is protected with the acid labile 1-ethoxyethyl (EE) group. The adoption of this method presented great selectivity in removing the 5'-hydroxyl protecting group and facilitated the RNA oligomer synthesis. A RNA pentamer was synthesized by the phosphotriester method in solution. |
| |
| Keywords: | |
|
|
