| β-鹅膏毒肽的反相高效液相色谱分离纯化* |
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| 引用本文： | 曹福祥,张志光,梁宋平.β-鹅膏毒肽的反相高效液相色谱分离纯化*[J].菌物学报,2003,22(1). |
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| 作者姓名： | 曹福祥 张志光 梁宋平 |
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| 作者单位： | 1.中南林学院生物化学教研室 2.湖南师范大学生命科学学院 |
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| 摘 要： | 用反相高效液相色谱,以0.02mol/L醋酸铵-乙腈为流动相的梯度洗脱模式,在295nm吸收值的条件下,灰花纹鹅膏菌Amanitafuliginea的肽类毒素可以被成功的分离和纯化。单个肽类毒素的鉴定是用反相高效液相色谱和质谱同时进行。用这一方法可从灰花纹鹅膏菌中分离纯化出β-鹅膏毒肽(β-amanitin),产量可达到:1158靏/g(干重),产品纯度达98%以上,回收率为95.3%。β-鹅膏毒肽的分子量为919.3Da。这个方法可用于其它鹅膏菌肽类毒素的分离纯化。
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| 关 键 词： | 肽类毒素 灰花纹鹅膏菌 质谱 |
ISOLATION AND PURIFICATION OF β-AMANITIN BY REVERSEDPHAGE HIGH-PERFORMANCE LIQUID CHROMATOGRAPH |
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| Abstract: | amanitin of Amanita fuliginea is isolated and purified by using reversed-phage high-performance liquid chromatograph under the condition elution of 0.02M aqueous ammoniumacetate-acetonitrile and absorbance at 295nm. Single toxin is determined by using reversed-phage high-performance liquid chromatograph and mass spectrum.The yield of β-amanitin may reach1214靏/g(dry-weight).The purities of the yield are over 98% and the recovery of yield is over 95.3%.The molecular weight is 919.3 dalton。Others peptide toxins of Amanita can also be isolated and purified by using this method. |
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| Keywords: | Peptide toxin Amanita fuliginea mass spectrum |
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