In vitro morphogenesis of pearl millet [Pennisetum glaucum (L.) R.Br.]: efficient production of multiple shoots and inflorescences from shoot apices |
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| Authors: | P Devi H Zhong M B Sticklen |
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| Institution: | (1) Department of Botany, Osmania University, Hyderabad, India, IN;(2) Novartis Seeds, Seed Biotech Research Unit, 3054 Cornvallis Road, Research Triangle Park, NC 27709, 3 Department of Botany, Osmania University, Hyderabad, India, IN;(3) Department of Crop and Soil Sciences and Entomology, 201 Center for Integrated Plant Systems, Michigan State University, East Lansing, MI 48824-1311, USA e-mail: sticklen@pilot.msu.edu Fax: +517-353-1698, US |
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| Abstract: | This report presents a procedure for high-frequency multiple shoot production from cultured shoot apical meristems of pearl
millet Pennisetum glaucum (L.) R. Br.]. Shoot apices from 1-week-old aseptically germinated seedlings were cultured in vitro on MS medium containing
various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyladenine (BA) with biweekly subculture. A low concentration
of 2,4-D coupled with four different concentrations of BA induced the production of adventitious shoots from the enlarged
shoot apical meristems. Somatic embryogenesis was also observed at higher concentrations of BA. The use of higher levels of
2,4-D resulted in callusing of shoot apical meristems, while the shoot tips produced many leaves and in vitro flowering in
2,4-D-free media containing BA. All four pearl millet genotypes produced similar results. Fertile pearl millet plants were
produced from in vitro-produced multiple shoots.
Received: 1 April 1999 / Revision received: 8 July 1999 / Accepted: 17 August 1999 |
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| Keywords: | Pennisetum glaucum (L ) R Br (pearl millet) In vitro Multiple shoots Meristems |
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