| 表达广谱抗真菌蛋白转基因水稻秸秆的化学成分与纤维素结构 |
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| 引用本文： | 宋永康,黄薇,姚清华,陈丽华,刘文静,李玥仁.表达广谱抗真菌蛋白转基因水稻秸秆的化学成分与纤维素结构[J].华东昆虫学报,2011(2):160-165. |
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| 作者姓名： | 宋永康 黄薇 姚清华 陈丽华 刘文静 李玥仁 |
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| 作者单位： | 福建省农业科学院中心实验室;福建省精密仪器农业测试重点实验室; |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项(2009ZX08011-029B) |
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| 摘 要： | 为了给表达广谱抗真菌蛋白转基因水稻的安全性评估提供基础数据,本文以表达广谱抗真菌蛋白转基因水稻转品1和转品8及非转基因七丝软粘(对照)为试材,对其秸秆化学成分进行分析,同时,采用傅立叶变换红外光谱(FTIR)和X-射线衍射(XRD)方法观察秸秆中不同部位纤维素结构的变化情况。研究结果显示:(1)转基因水稻转品1和转品8与对照在秸秆相同部位的化学成分含量不存在显著差异;(2)转基因水稻转品1和转品8及对照在秸秆相同部位纤维素的红外吸收峰形状基本一致,且品种间的吸收峰强度和结晶指数(O'KI和N·O'KI)也无显著差异;(3)转品1和转品8秸秆的X-射线衍射图与对照相似,且结晶度(CrI)与对照无显著差异。综上所述,广谱抗真菌基因的导入不会对水稻的化学成分、纤维素结构及晶体结构产生明显影响。
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| 关 键 词： | 转基因水稻 秸秆 化学成分 纤维素结构 |
Chemical composition and cellulosic structure of straw from transgenic rice (Oryza sativa) plants expressing broad-spectrum antifungal proteins |
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| Yong-kang SONG,Wei HUANG,Qing-hua YAO,Li-hua CHEN,Wen-jing LIU,Yue-ren LI, Central Laboratory,Fujian Academy of Agricultural Sciences,Fujian Key Laboratory of Precise Measurement of Agricultural,Fuzhou,Fujian ,China.Chemical composition and cellulosic structure of straw from transgenic rice (Oryza sativa) plants expressing broad-spectrum antifungal proteins[J].Entomological Journal of East China,2011(2):160-165. |
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| Authors: | Yong-kang SONG Wei HUANG Qing-hua YAO Li-hua CHEN Wen-jing LIU Yue-ren LI Central Laboratory Fujian Academy of Agricultural Sciences Fujian Key Laboratory of Precise Measurement of Agricultural Fuzhou Fujian China |
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| Institution: | Yong-kang SONG1,2,Wei HUANG1,Qing-hua YAO1,Li-hua CHEN1,Wen-jing LIU1,Yue-ren LI1,2 1Central Laboratory,Fujian Academy of Agricultural Sciences,2Fujian Key Laboratory of Precise Measurement of Agricultural,Fuzhou,Fujian 350003,China |
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| Abstract: | Using two transgenic rice (Oryza sativa) plant lines, Zhuanpin 1 and Zhuanpin 8, expressing broad-spectrum antifungal proteins and their non-transgenic parent cv.Qisiruanzhan as materials, the chemical composition of straw was analyzed, and the changes of cellulose in different parts of straw were studied by fourier transform infrared spectroscopy (FTIR) and X-ray diffractometer (XRD). Infrared absorption spectra of cellulose from leaf blade, leaf sheath, and stem was basically the same in the transgenic vs... |
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| Keywords: | transgenic rice straw chemical composition cellulose structure |
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