福建闽侯福橘黑斑病病原菌的鉴定

柑橘黑斑病是柑橘的重要病害之一。本文通过病原菌的形态特征和致病性,并结合其rDNA-ITS区域序列及柯赫法则,对从福建省福州市闽侯县福橘果上分离的黑斑病病原菌进行了鉴定分析。结果表明:PDA平板上的病原菌菌落生长缓慢,呈圆形凸起;分生孢子器近球形、黑色炭质;分生孢子单孢、无色,呈椭圆形,大小为(7～12)μm×(5～8)μm,分生孢子外包裹一层半透明凝胶状物质。结合序列比对分析结果,最终将分离的福橘果实黑斑病的病原菌鉴定为柑橘球座菌Guig-nardia citricarpa Kiely。本研究为柑橘黑斑病的早期诊断和防治奠定了基础。

Identification of the pathogen causing citrus black spot disease on Citrus reticulate Blanco cv.Tangerina fruit in Minhou, Fujian Province

Citrus black spot disease is one of the important diseases on citrus. In this paper, the pathogen causing the citrus black spot disease was identified on Citrus reticulate Blanco cv.Tangerina in Minhou County, Fujian Province, based on Koch′s postulates and rDNA internal transcribed spacer（ITS） sequences. The sample was collected on July 2009. The colony growing slowly from the PDA medium plate was round and protuberant. The spherical pycnidium was black and the monadic conidiophore elliptic, colourless, （7~12） μm×（5~8） μm, surrounded by a layer of translucent gelatinous coat. The ITS sequence analysis proved that the pathogen was Guignardia citricarpa Kiely. This is the fourth confirmed record of this pathogen from China. This research laid a foundation for early diagnosis and treatment of citrus black spot disease.