Subcellular distributions of calcium/calmodulin-stimulated and guanine nucleotide-regulated adenylate cyclase activities in the cerebral cortex |
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| Authors: | Shirley T Bissen Tetsufumi Ueda |
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| Institution: | (1) Department of Pharmacology, The University of Michigan, 48109 Ann Arbor, Michigan;(2) Department of Psychiatry, The University of Michigan, 48109 Ann Arbor, Michigan;(3) Mental Health Research Institute, The University of Michigan, 48109 Ann Arbor, Michigan;(4) Present address: Department of Zoology, University of California, 94720 Berkeley, CA |
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| Abstract: | The subcellular distribution of Ca2+/calmodulin-stimulated adenylate cyclase activity was studied in comparison with that of guanine nucleotide-stimulated cyclase activity. The distributions of these activities were similar among the crude fractions but differed among the purified subsynaptosomal fractions. The specific activity of Ca2+/calmodulin-stimulated cyclase was highest in a light synaptic membrane fraction, which has few, if any, postsynaptic densities, whereas that of guanine nucleotide-stimulated cyclase was highest in a heavier synaptic membrane fraction rich in postsynaptic densities. These results suggest that the Ca2+/calmodulin-stimulated cyclase has, at least in part, a different cellular or subcellular location than the guanine nucleotide-stimulated cyclase.Abbreviations used CaM
calmodulin
- GppNHp
guanosine 5 -( , -imino) triphosphate |
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