质谱流式技术用于单细胞检测

质谱流式技术(mass cytometry)是利用质谱原理对单细胞进行多参数检测的流式技术,能够在单细胞水平实现超过50种标志物的同时测量,显著增强了对细胞生长进程和复杂细胞系统的评估能力。该文简要介绍了质谱流式技术的基本工作原理,并从金属元素标记、质量分析器、高维单细胞数据处理等方面展开论述,阐明设计新型金属元素标签和选择飞行时间质谱的必要性,归纳分析高维单细胞数据的算法并总结各种算法的优点和局限性。

Application of Mass Cytometry in Single-Cell Detection

Mass cytometry is a cytometric technique in which multiple biomarkers of a single cell are detected through mass spectrometry.It can simultaneously measure more than 50 biomarkers at single-cell resolution,effectively augmenting the ability to evaluate cell growth processes and complex cellular systems.This work will briefly introduce the basic principle of mass cytometry and discuss metal labeling,mass analyzer and high-dimensional single-cell data processing.The needs to design new metal tags and select time-of-flight mass spectrometry are clarified,the algorithms which can be used for high-dimensional single-cell data analysis are described.At the same time,the advantages and limitations of these algorithms are discussed.