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| 用非放射性原位杂交在同一张切片上同时在光学和电子显微镜下显示的豌豆rDNA的分布(简报) | |
| 引用本文： | 王新宇,聂秀菀,M.I.Mighett,D.J.Rawlins,P.J.Shaw. 用非放射性原位杂交在同一张切片上同时在光学和电子显微镜下显示的豌豆rDNA的分布(简报)[J]. 分子细胞生物学报, 1996, 0(1) |
| 作者姓名： | 王新宇 聂秀菀 M.I.Mighett D.J.Rawlins P.J.Shaw |
| 作者单位： | 兰州大学细胞生物学研究室，兰州大学细胞生物学研究室，John Innes Institute of Plant Sciences Norwich，NR 47 UH，UK，John Innes Institute of Plant Sciences Norwich，NR 47 UH，UK，John Innes Institute of Plant Sciences Norwich，NR 47 UH，UK 兰州 730000，兰州 730000 |
| 摘 要： | 原位杂交目前已成为研究各类组织或细胞内DNA或RNA定位最有效的手段,特别是在发育生物学领城,对于研究基周的表达和修饰更具有重要意义。旱期的原位杂交主要是在 |
| 关 键 词： | 非放射性原位杂交 间期细胞核 显微和亚显超微结构 豌豆 |
LOCALIZATION OF PISUM SATIVUM rDNA SIMULTANEOUSLY AT LEVELS OF LIGHT AND ELECTRON MICROSCOPY BY NON-RADIOACTIVE IN SITU HYBRIDIZATION |
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| Wang Xin-yu Nie Xiu-wan. LOCALIZATION OF PISUM SATIVUM rDNA SIMULTANEOUSLY AT LEVELS OF LIGHT AND ELECTRON MICROSCOPY BY NON-RADIOACTIVE IN SITU HYBRIDIZATION[J]. Journal of Molecular Cell Biology, 1996, 0(1) | |
| Authors: | Wang Xin-yu Nie Xiu-wan |
| Abstract: | A digoxigenin (DIG)-labelled rRNA probe was used to hybridize in situ to the vibration sections of paraformaldehyde fixed root tips, followed by immunogold staning and silver enhancement treatment After the vibration sections which had undergone in situ hybridization, immunogold staining and silver enhancement were observed and photographed under the light microscope, they were further prepared into ultrathin sections and studied underthe electron-microscope. This method more directly combined the in situ hibridization at level of the light microscope with that at level of the electron microscope, by using a piece of vibration section, through which a more detailed rDNA localization was revealed in interphase nuclei of Pisum sativum. Some advantage and disadvantage about the method is discussed in this paper. |
| Keywords: | non-radioactive in situ hybridization celluar and subcellular structure. Pisum sativum. interphase nuclei. |
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