Analysis of polymorphic DNA markers cosegregation with arterial blood pressure in ISIAH hypertensive rats] |
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| Authors: | A A Krivenko M L Filipenko V A Adarichev L N Maslova V V Bulygina G M Dymshits A L Markel' |
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| Institution: | Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, Russia. |
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| Abstract: | A search for DNA markers of hereditary arterial hypertension in ISIAH rats was performed by means of contemporary molecular genetic approaches. The backcross rat population used for the analyses was derived from a cross of the Wistar x ISIAH F1 progeny with the Wistar rats. Hybridization of the HaeIII-digested DNA samples with the (CAC)5 microsatellite probe revealed cosegregation of the basal arterial pressure value with the 4.8-kb polymorphic DNA fragment. Examination of the DNA polymorphism by means of polymerase chain reaction with arbitrary primers showed an association of the 700-bp polymorphic DNA fragment with the increase of arterial blood pressure under conditions of emotional stress. |
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