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Li+ effect on the cell wall of the yeast Saccharomyces cerevisiae as probed by FT-IR spectroscopy |
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| Authors: | Aurelijus Zimkus Audrius Misiūnas Larisa Chaustova |
| Affiliation: | 1186. Department of Biochemistry and Biophysics, Vilnius University, LT-01513, Vilnius, Lithuania 2186. Department of Organic Chemistry, Center for Physical Sciences and Technology, LT-01108, Vilnius, Lithuania 3186. Department of Bioelectrochemistry and Biospectroscopy of Institute of Biochemistry, Vilnius University, LT-01513, Vilnius, Lithuania |
| Abstract: | The effect of Li+ ions as a transformation inducing agent on the yeast cell wall has been studied. Two Saccharomyces cerevisiae strains, p63-DC5 with a native cell wall, and strain XCY42-30D(mnn1) which contains structural changes in the mannan-protein complex, were used. Fourier transform infrared (FT-IR) spectroscopy has been used for the characterization of the yeast strains and for determination of the effect of lithium cations on the cell wall. A comparison of the carbohydrate absorption band positions in the 970–1185 cm?1 range, of Na+ and Li+ treated yeast cells has been estimated. Absorption band positions of the cell wall carbohydrates of p63-DC5 were not influenced by the studied ions. On the contrary, the treatment of XCY42-30D(mnn1) cells with Li+ ions shifted glucan band positions, implying that the cell wall structure of strain XCY42-30D(mnn1) is more sensitive to Li+ ion treatment. |
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