Peroxidase activity and isoenzymes in the culture medium of NaCl adapted tomato suspension cells |
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Authors: | María A Sancho Silvia Milrad de Forchetti Fernando Pliego Victoriano Valpuesta Miguel A Quesada |
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Institution: | (1) Departamento de Biología Vegetal, Universidad de Málaga, 29071 Málaga, Spain;(2) Dept. de Bioquímica y Biología Molecular, Universidad de Málaga, 29071 Málaga, Spain |
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Abstract: | The medium of tomato (Lycopersicon esculentum) cells adapted to grow in the presence of 15 g l–1 NaCl had a higher peroxidase activity than the medium of an unadapted tomato cell line. When the adapted cells were cultured in a medium without NaCl, the value found for peroxidase activity was intermediate. The increase in peroxidase activity was parallel to an increase of lignin-like compounds in the cell walls, as well as to an increased content or appearance of neutral and basic peroxidase isoenzymes. Apparently, the high values of peroxidase activity in the medium of the salt-adapted cells reflect the changed mechanical properties of the cell wall which, in turn, could be related to the salt adaptation process.Abbreviations LO
Control tomato cell line unable to grow in the presence of 15 g 1–1 of NaCl
- L15
tomato cell line adapted to 15 g 1–1 of NaCl and growing in this salt concentration
- L15-0
tomato cell line adapted to 15 g 1–1 of NaCl and growing in the absence of this salt
- 2,4-D
2,4-dichlorophenoxyacetic acid
- NAA
naphthaleneacetic acid
- PBS
phosphate buffer saline |
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Keywords: | Lignin Lycopersicon esculentum salt-stress peroxidase isozymes |
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