Somatic embryogenesis and plant regeneration from different wild diploid cotton (Gossypium) species |
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Authors: | Yuqiang Sun Xianlong Zhang Chao Huang Xiaoping Guo Yichun Nie |
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Institution: | (1) National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, P.R. China |
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Abstract: | Calli were successfully induced from hypocotyls of eight wild diploid cotton species (Gossypium) on MSB (MS salts and B5 vitamins) medium supplemented with 0.09 μM 2,4-D (2,4-dichlorophenoxyacetic acid) and 2.32 μM KT (kinetin). Plant growth
regulator (PGR) combinations, adding GA3 (Gibberellic acid), high inorganic salt stress, and PGR-free media were used to induce embryogenic calli from nonembryogenic
calli. Embryogenic cultures were induced from G. aridum S. (D4 genome), G. davidsonii K. (D3-d genome), G. klotzschianum A. (D3-k genome), G. raimondii U. (D5 genome), and G. stocksii M. (E1 genome). We then observed somatic embryogenesis in the five species while calli of G. africanum V. (A1-2 genome), G. anomalum W. (B1 genome), and G. bickii P. (G genome) remained nonembryogenic. Somatic embryogenesis was adjusted by changing sugar sources, regulating combinations
of PGRs, and using cell suspension culture. Embryos at various developmental stages produced mature and germinating embryos
when cultured on filter paper placed on the media containing different sugar sources. The utility of different sugar sources
promoted globular embryos developing into cotyledonary stage and increased the frequency of cotyledonary embryos developing
into normal plants. Normal plantlets were regenerated from G. davidsonii, G. klotzschianum, G. raimondii, and G. stocksii. Only abnormal plantlets were obtained in G. aridum. This work will contribute to broadening the number of regenerable cotton species and provide foundations for somatic hybridization
in cotton to create new germplasm. |
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Keywords: | Differentiation Germplasm Gossypium Plant growth regulation Plant regeneration Wild diploid cotton |
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