Application of the Papanicolaou Stain to Paraffin Sections |
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Authors: | Percy L Johnson Morris N Klein |
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Institution: |
a Department of Histology, College of Dentistry and, The Murray and Leonie Guggenheim Institute for Dental Research of New York University, New York, NY |
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Abstract: | Routine paraffin sections from tissues fixed either in aqueous formalin, 80% alcohol (with or without 1% trichloracetic acid added), Carnoy's alcohol-chloroform-acetic (6:3:1) and Bouin's fixative were stained as follows: Harris' hematoxylin, 6 min; running water, 2-3 min; ascending grades of alcohol to 95%; orange G, 0.5% and phosphotungstic acid, 0.015% in 95% alcohol, 5 min; 95% alcohol, 2 changes; Papanicolaou's EA36, 2.5 min; dehydration, clearing, and covering in Permount. The results show morphology better than hematoxylin and eosin and the technic is recommended particularly for keratin, which always stains bright orange. |
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