Identification and Characterization of a cDNA Clone Encoding the Heat Shock Protein (Hsp60) from the Biting Midge, Culicoides variipennis sonorensis Wirth and Jones |
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| Authors: | M A Abdallah R S Pollenz R A Nunamaker K E Murphy |
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| Institution: | (1) Department of Microbiology and Molecular Cell Sciences, The University of Memphis, Memphis, Tennessee, 38152.;(2) Present address: Department of Anesthesiology, University of California at San Francisco, San Francisco, California, 94143;(3) Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina, 29406;(4) Arthropod-Borne Animal Diseases Research Laboratory, USDA-ARS, Laramie, Wyoming, 82071 |
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| Abstract: | A cDNA expression library constructed from Culicoides variipennis sonorensis was screened using an antibody specific for Hsp60 of Heliothis virescens. A single clone encoding the complete heat shock protein (Hsp60) of C. variipennis was identified and its 2400-bp insert was sequenced. The encoded 62-kDa protein contains 581 amino acids and includes a 26-amino acid putative mitochondrial targeting sequence at its N terminus and a GGM motif at its carboxyl terminus. Deduced amino acid sequences are highly similar (67–78%) to Hsp60 of other species, including the fruit fly, the house mouse, the Norwegian rat, the Chinese hamster, the human, a nematode, and the tobacco budworm moth. This is the initial isolation of a coding sequence for a stress-induced protein in C. variipennis. |
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| Keywords: | hsp60 cDNA Culicoides variipennis chaperonin |
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