Differential display reveals a novel pig gene,PRPF3, which is differentially expressed in Large White versus Wujin skeletal muscle tissues |
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Authors: | Liu Yonggang |
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Institution: | (1) College of Animal Sciences and Technology, Yunnan Agricultural University, 650201 Kunming, China |
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Abstract: | The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi
muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete
cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends method. The open reading frame of this gene
encodes a protein of 683 amino acids, which is homologous with the PRP3 pre-mRNA processing factor 3 (PRPF3) of five species:
bovine (99%), human (99%), sumatran orangutan (99%), mouse (99%) and chicken (94%). This newly identified gene was respectively
defined as the swine PRPF3 gene. This gene is structured in 15 exons and 14 introns as revealed by computer-assisted analysis.
The phylogenetic tree analysis revealed that the swine PRPF3 gene has a closer genetic relationship with the PRPF3 gene of
sumatran orangutan. |
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Keywords: | |
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