A silver stain for the detection of nanogram amounts of tRNA following two-dimensional electrophoresis |
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| Authors: | G L Igloi |
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| Institution: | 1. Department of Biochemistry, School of Medicine, Oregon Health Sciences University, Portland, Oregon 97201, USA;2. Department of Epidemiology and International Health, University of California Medical School, San Francisco, California 94143 USA |
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| Abstract: | Three ultrasensitive protein silver-staining methods have been compared with respect to the detection of tRNA in polyacrylamide gels. The method of Sammons (D. W. Sammons, L.D. Adams, and E.E. Nishizawa (1981) Electrophoresis 2, 135-141) has been shown to have remarkable sensitivity, with a detection limit of 0.3 ng tRNA/mm2, allowing the two-dimensional fractionation of submicrogram amounts of bulk tRNA. The application of this technique to developmental and differentiation problems and other areas where the amounts of nonradioactive tRNA available are limited is anticipated. |
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| Keywords: | nanogram amounts of tRNA silver stain two-dimensional gel electrophoresis |
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