Effects of neuropeptide F on regeneration in <Emphasis Type="Italic">Girardia tigrina</Emphasis> (Platyhelminthes) |
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Authors: | Natalia D Kreshchenko Zakhar Sedelnikov Inna M Sheiman Maria Reuter Aaron G Maule Margaretha K S Gustafsson |
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Institution: | Institute of Cell Biophysics, Pushchino, Moscow Region, Russia. |
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Abstract: | The effects of neuropeptide F (NPF; from Moniezia expansa) on the regeneration of Girardia tigrina were studied. The animals were decapitated and incubated in water (control) or NPF. The dynamics of the proliferation of
the neoblasts in the developing tissue were studied during the course of regeneration by monitoring the mitotic index (MI).
The effects of incubation in FMRFamide and GYIRFamide on the MI were also tested. The course of cephalic regeneration was
followed with in vivo computer-assisted morphometry for up to 7 days. The development of the regenerating nervous system and
the musculature was visualised by immunostaining with a primary antiserum to the C-terminal decapeptide of NPF (YFAIIGRPRFa)
and tetramethylrhodamine-isothiocyanate-conjugated phalloidin, which stains F-actin in muscle filaments. The study showed
that NPF had a stimulatory effect on the mitotic activity of the neoblasts. FMRFamide and GYIRFamide did not have this effect.
NPF also stimulated the growth of the regenerating head and the growing nervous system and musculature. NPF is postulated
to have a morphogenetic action in the regenerating animals.
This work was supported by two grants from the Finnish Academy of Science (nos. 202685, 2004) and (no. 112090, 2006) to M.G.,
an RFBR grant (07-04-00452a) to N.K. and a Wellcome Trust grant (069411) to A.G.M. for which we express our gratitude. |
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Keywords: | Regeneration Neoblasts Mitotic index Neuropeptides Girardia tigrina (Platyhelminthes) |
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