A replica plating technique for the study of plaque-forming centers |
| |
| Authors: | I Rappaport |
| |
| Affiliation: | Department of Microbiology, New York Medical College, New York, New York 10029 U.S.A. |
| |
| Abstract: | Mouse spleen cells were cultured on Millipore filters supported on the surface of Eagle's agarose medium. The secretory products of individual plaque-forming centers (PFC) revealed after diffusion into the agarose, were studied over an extended period of time by transferring the Millipore filters to fresh agarose surfaces repeatedly in a manner analogous to the replica plating technique employed with bacteria. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
