利用生化及分子标记确定长穗偃麦草(Elytrigia elongatum, EE. 2n=14)染色体与小麦染色体的部分同源性

利用限制性片段长度多态性（ＲＦＬＰ）及等电聚焦（ＩＥＦ）技术确定普通小麦中国春－二倍体长穗僵麦草７个异附加系所附加的外源染色体与小麦染色体的部分同源性，共有８个生化标记，１３个ＲＦＬＰ标记在亲本间揭示了多态性。结果表明：长穗堰麦草的ＩＥ、２Ｅ、３Ｅ、４Ｅ、 ５Ｅ、６Ｅ、７Ｅ ７条染色体分别与小麦染色体的 １、２、３、４、５、６、７ ７个部分同源群具有部分同源关系，堰麦草的ＩＥ与７Ｅ、５Ｅ与７Ｅ染色体间可能发生过重排。同时，研究还分别将Ｅｓｔ－Ｅ５、Ｅｓｔ－Ｅ８位点定位于３ＥＬ，Ｐｅｒ－Ｅ１定位于７Ｅ， Ｐｅｒ－Ｅ４定位于５Ｅ，β－Ａｍｙ－Ｅ１定位于４ＥＬ染色体，并进一步将α－Ａｍｙ－Ｅ１位点定位于６Ｅ染色体长臂上。

Identification of Homoeology between the Elytrigia elongatum (2n=14, EE) and Wheat Chromosomes Using Biochemical and Molecular Markers

Restriction fragment length polymorphism (RFLP) and Isoelectric focusing (IEF) were used to identify the homoeology between Elytrigia elongatum and wheat chromosomes. There were eight biochemical and thirteen RFLP markers Showing polymorphism between the two parents. The result indicated that the chromosomes of 1E, 2E, 3E, 4E, 5E, 6E, 7E were homoeologous to the lst, 2nd, 3rd, 4th, 5th, 6th, 7th homoeologous groups, respectively. Chromosome translocation between 1E and 7E, 5E and 7E took place probably during the evoluation process of E genome. Meanwhile, five biochemical markers such as Est-E5, Est-E8, Per-E1, Per-E4 and -Amy-E1 were located in E genome chromosomes, the -Amry-E1s loci was relocated in 6EL