| 一株纤维素降解真菌的筛选、鉴定及酶学性质分析 |
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| 引用本文： | 甄静,王继雯,谢宝恩,李冠杰,刘莹莹,周伏忠,陈国参.一株纤维素降解真菌的筛选、鉴定及酶学性质分析[J].微生物学通报,2011,38(5):709-714. |
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| 作者姓名： | 甄静 王继雯 谢宝恩 李冠杰 刘莹莹 周伏忠 陈国参 |
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| 作者单位： | 1. 河南省微生物工程重点实验室,河南,郑州,450008;河南省科学院生物研究所有限责任公司,河南,郑州,450008
2. 河南省微生物工程重点实验室,河南,郑州,450008 |
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| 基金项目： | 2009年河南省重大公益科研招标项目(No. 091100910500) |
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| 摘 要： | 通过对富含枯枝败叶的土壤样品进行富集培养,利用刚果红纤维素培养基初筛和酶活测定复筛得到产纤维素酶的一株真菌,将其命名为GC2-2,并对该菌株进行鉴定及酶学性质研究。结果表明该菌株是一株耐高温、碱性纤维素酶的真菌GC2-2。通过18S rDNA分子克隆测定,该菌为球孢枝孢菌,其滤纸酶的活力优于CMC酶的活力。该菌所产酶的最适反应条件为温度35°C,最适pH值7.5。
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| 关 键 词： | 真菌 球孢枝孢菌 碱性纤维素酶 |
Isolation, identification of a cellulase-producing strain and characterization of its cellulase-producing capability |
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| ZHEN Jing,WANG Ji-Wen,XIE Bao-En,LI Guan-Jie,LIU Ying-Ying,ZHOU Fu-Zhong and CHEN Guo-Can.Isolation, identification of a cellulase-producing strain and characterization of its cellulase-producing capability[J].Microbiology,2011,38(5):709-714. |
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| Authors: | ZHEN Jing WANG Ji-Wen XIE Bao-En LI Guan-Jie LIU Ying-Ying ZHOU Fu-Zhong and CHEN Guo-Can |
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| Institution: | 1. Key Laboratory of Microbial Engineering of Henan, Zhengzhou, Henan 450008, China; 2. Henan Academy of Sciences Institute of Biology Co., Ltd, Zhengzhou, Henan 450008, China;1. Key Laboratory of Microbial Engineering of Henan, Zhengzhou, Henan 450008, China; 2. Henan Academy of Sciences Institute of Biology Co., Ltd, Zhengzhou, Henan 450008, China;1. Key Laboratory of Microbial Engineering of Henan, Zhengzhou, Henan 450008, China; 2. Henan Academy of Sciences Institute of Biology Co., Ltd, Zhengzhou, Henan 450008, China;1. Key Laboratory of Microbial Engineering of Henan, Zhengzhou, Henan 450008, China; 2. Henan Academy of Sciences Institute of Biology Co., Ltd, Zhengzhou, Henan 450008, China;1. Key Laboratory of Microbial Engineering of Henan, Zhengzhou, Henan 450008, China; 2. Henan Academy of Sciences Institute of Biology Co., Ltd, Zhengzhou, Henan 450008, China;1. Key Laboratory of Microbial Engineering of Henan, Zhengzhou, Henan 450008, China;1. Key Laboratory of Microbial Engineering of Henan, Zhengzhou, Henan 450008, China |
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| Abstract: | A new fungus, designated GC2-2, which produced thermostable alkaline cellulase was isolated from deadwood soil. GC2-2 was identified as Cladosporium sp. by morphological characteristics as well as by analysis of the gene encoding the 18S rRNA. Cladosporium sp. GC2-2 produced Cellulase enzyme. The enzyme activity on filter paper was higher than that on CMC. The optimal conditions for the enzymatic reaction were about 35 °C and at pH 7.5. |
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| Keywords: | Fungi Cladosporium sp Alkaline-cellulase |
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