用免疫荧光单克隆抗体对脊髓灰质炎病毒抗原表位的分析

用间接免疫荧光与中和试验筛选出来的抗脊髓灰质炎2型与3型不同毒株的12个单克隆抗体,其中3个仅有免疫荧光活性,9个具有中和与免疫荧光活性。用免疫荧光活性的单克隆抗体进行试验,发现它们在识别特异性抗原表位方面与中和性单克隆抗体相似,显示出株特异的、几个毒株共同特异的或型特异的抗原表位。根据表位分布关系及特征,可以用来鉴别型内毒株的特征、毒株的抗原分析、抗原变异的研究以及疫苗相关病例的鉴别。所得结果与中和性单克抗隆抗体及T1-寡核苷酸指纹图谱分析一致。而免疫荧光单克隆抗体识别抗原表位的活性范围比中和性单克隆抗体更广。另外还发现某些兼有荧光与中和两活性的同一个单克隆抗体,用不同方法(IF与NT)进行试验时,与相同毒株出现不同表位反应,这点是值得引起注意需待进一步证实的重要问题。

Analysis of Poliovirus Antigenic Epitopes by Immunofluorescence Activated McAbs

12 McAbs to several strains of poliovirus Type 2 and 3 were selected by IF and NT, 9 McAbs had both neutralizing and immunofluorescence activity,3 McAbs were uniquely with IF staining activity. All the 12 McAbs with immunofluorescence activity reacted with poliovirus Type 2 and 3(F-McAbs) and were found similar to neutranizing McAbs (N-McAbs) recognizing different spesific antigenic epitopes on infected cells. According to topological relationships of antigenic epitopes, it was possible to characterize the virus, identify the interatypic differentiation of poliovirus strains, analyse the antigenic variation and recognize the vaccine related strains. The results were similar to neutralization and T1-oligonucleotide map. There was a broade detecting activity range in IF staining than in neutralization testing. For example, between Finland/23127/84 and Yu/2/84 s rains,which were isolated during an outbreak of polimyelitis in Finland and in China respectively, related antigenic epitope was not observed in NT, but was found in IF staining. Some of McAbs showed different reactions with the same strains in IF and NT This was an important problem that should be further studied. F-McAbs prove to be a very valuable reagents for diagnosing, identifying and analysing kits of McAbs.