13C and31P NMR spectroscopic studies on glucose metabolism inTribolium confusum |
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| Authors: | M. Nishina K. Matsushita E. Hori M. Takahashi K. Kato |
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| Affiliation: | (1) Department of Medical Zoology, Saitama Medical School, 38 Morohongo Moroyama, 350-04 Truma-gun, Saitama, Japan;(2) Department of Medical Entomology, National Institute of Health, Shinagawa-ku, 141 Tokyo, Japan;(3) Department of Enteroviruses, National Institute of Health, Shinagawa-ku, 141 Tokyo, Japan |
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| Abstract: | Nuclear magnetic resonance (NMR) technology was applied to study the glucose metabolism inTribolium confusum (Coleoptera).13C signals of D-(1-13C)glucose eaten by beetles were clearly detected in such metabolites of the glucose metabolism as glycogen, trehalose, triacylglycerol, alanine and proline by13C-NMR. After glucose feeding the31P-NMR spectra ofT. confusum showed the signal intensity increases in arginine-phosphate, sugar-phosphate and uridine diphosphoglucose. The results demonstrated the potential of NMR analysis for the study of glucose metabolism inT. confusum. |
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| Keywords: | 13C-NMR 31P-NMR Nuclear magnetic resonance Tribolium confusum Glucose metabolism |
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