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Metabolism of Gibberellins in Cell-Free Extracts of Anthers from Normal and Dwarf Rice |
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| Authors: | Kobayashi Masatomo; Kamiya Yuji; Sakurai Akira; Saka Hitoshi; Takahashi Nobutaka |
| Institution: | 1The Institute of Physical and Chemical Research Wako-shi, Saitama, 351-01 Japan 2National Institute of Agrobiological Resources Tsukuba-shi, Ibaraki, 305 Japan 3Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo, 113 Japan |
| Abstract: | Cell-free extracts were prepared from anthers of normal anddwarf rice (Oryza sativa L.), and the metabolism of radioisotope-labeledgibberellins in the extracts was analyzed by HPLC and gas chromatography-massspectrometry (GC/MS). GA12 was converted to GA15 and GA34 inthe extracts. GA20 was converted to GA1, GA8 and GA29, but GA9was converted only to GA34. The extracts of the dwarf cultivar,Waito-C (dy mutant), showed the same 3ß-hydroxylationactivity as did those of the normal cultivar, Nihonbare, indicatingthat the dy gene is not expressed in the anthers. These resultssuggest that the regulation of the biosynthesis of gibberellinsin rice is organ-specific. (Received November 9, 1989; Accepted January 10, 1990) |
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