| C．shehatae TZ8耐乙醇菌株的筛选及发酵性能研究 |
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| 引用本文： | 周逢云,汤斌,张庆庆,翟光雯,陈阿娜.C．shehatae TZ8耐乙醇菌株的筛选及发酵性能研究[J].生物学杂志,2009,26(1):27-30. |
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| 作者姓名： | 周逢云 汤斌 张庆庆 翟光雯 陈阿娜 |
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| 作者单位： | 安徽工程科技学院生化工程系,微生物发酵安徽省工程技术研究中心,芜湖241000 |
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| 基金项目： | 安徽省高校自然科学基金重点项目 |
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| 摘 要： | 以C.shehataeTZ8为出发茵株，利用1％溶壁酶和1％蜗牛酶酶解1．5h，制备成C.shehataeTZ8原生质体，并对原生质体进行紫外诱变，以含不同浓度乙醇的木糖液体培养基培养进行初筛和复筛，获得一株遗传性能稳定、耐乙醇能力达5．5％（v／v）的蕾株C．shehataeTZ8-4，比初始菌株耐乙醇能力提高了2％。对突变株C．shehataeTZ8-4发酵性能的研究结果表明：C．shehataeTZ8-4发酵糖能力从80g/L（葡糖糖和木糖比为2：1）提高到120g／L，最大乙醇产量从27．41g／L提高到43．12g／L。
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| 关 键 词： | 原生质体诱变 休哈塔假丝酵母 乙醇 发酵 |
Screening of alcohol tolerant Candida shehataeand its fermentation characteristics |
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| ZHOU Feng-yun,TANG Bin,ZHANG Qing-qing,ZHAI Guang-wen,CHEN Ana.Screening of alcohol tolerant Candida shehataeand its fermentation characteristics[J].Journal of Biology,2009,26(1):27-30. |
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| Authors: | ZHOU Feng-yun TANG Bin ZHANG Qing-qing ZHAI Guang-wen CHEN Ana |
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| Institution: | 1. Department of Biochemical Engineering,Anhui University of Technology and Science,2. Engineering and Research Center of Microbial Fermentation, Wuhu 241000, China) |
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| Abstract: | The protoplasts of C. shehataeTZ8 were obtained by lywallzyme and snail enzymes. Lywallzyme and snail enzymes concentration was both 1%. Enzymatic hydrolysis time was 1.5h. Ultraviolet radiation was used to mutagenize the protoplasts of Candida shehataeTZ8 for screening high ethonal concentration tolerance strain. Combining further screening experiments on cultivation with different ethanol yield in xylose-culture, a mutant strain C. shehataeTZS-4 was eventually obtained. Its alcohol tolerance degrading achieved 5.5% and increased by 2% compared with that of original strain. The genetic property of mutant strain was genetic stable Its ability of degrading glucose/xylose mixtures （ G： X =2：1 ） was improved from 80 g/L to 120 g/L Ethanol yield increased from 27.41 g/L to 43.12 g/L |
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| Keywords: | protoplasts mutagensis Candida shehatae fermentation ethanol |
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