Stimulation of the growth and solamargine production by Solanum paludosum multiple shoot cultures using a new culture medium |
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Authors: | H El Badaoui P Morard M Henry |
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Institution: | (1) Laboratoire de Botanique et Mycologie, Faculté des Sciences Pharmaceutiques et Biologiques, 5, Rue Albert Lebrun, B.P. 403, 54001 Nancy cedex, France;(2) Laboratoire d'Ingéniérie agronomique, Ecole Nationale Supérieure d'Agronomie, 45, avenue de Muret, 31000 Toulouse, France |
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Abstract: | Organogenic callus cultures of Solanum paludosum were obtained from root, hypocotyle and cotyledon explants of plantlets cultured in sterile conditions. These callus cultures developed multiple shoots which proliferated in Murashige and Skoog basal liquid medium. These multiple shoots produced solamargine, the main steroidal glycoalkaloid present in the unripe fruits.The optimization of the macronutrient composition of the liquid medium was performed by a method derived from the plant composition. This approach results in the establishment of an appropriate medium (SPOM medium) suitable for the improvement of both growth and solamargine production by multiple shoot cultures of S. paludosum. |
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Keywords: | multiple shoot culture macronutrient optimized culture medium solamargine production Solanum paludosum |
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