| 糜酶基因在转染的乳鼠心脏组织细胞中的表达 |
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| 引用本文： | 何泉,陈兰英. 糜酶基因在转染的乳鼠心脏组织细胞中的表达[J]. 中国生物化学与分子生物学报, 1998, 14(2): 227-229 |
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| 作者姓名： | 何泉 陈兰英 |
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| 作者单位： | 中国医学科学院(何泉)，中国协和医科大学(陈兰英) |
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| 基金项目： | 国家自然科学基金,中国医科院基金 |
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| 摘 要： | 糜酶基因在转染的乳鼠心脏组织细胞中的表达何泉陈兰英（中国医学科学院、中国协和医科大学，心血管病研究所、阜外心血管病医院，北京１０００３７）ＴｈｅＨｕｍａｎＨｅａｒｔＣｈｙｍａｓｅＧｅｎｅＥｘｐｒｅｓｓｉｏｎｉｎＣｕｌｔｕｒｅｄＮｅｏｎａｔａｌＲａｔＨ...
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| 收稿时间： | 1998-04-20 |
The Human Heart Chymase Gene Expression in Cultured Neonatal Rat Heart Tissue Cells |
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| He Quan Chen Lan Ying. The Human Heart Chymase Gene Expression in Cultured Neonatal Rat Heart Tissue Cells[J]. Chinese Journal of Biochemistry and Molecular Biology, 1998, 14(2): 227-229 |
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| Authors: | He Quan Chen Lan Ying |
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| Affiliation: | (Cardiovascular Institute and Fu Wai Hospital,CAMS and PUMC,Beijing 100037 |
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| Abstract: | In order to investigate the expression and regulation of human heart chymase gene in mammalian cardiac tissue,the neonatal rat myocardial and nonmyocardial cells in cardiac tissue were cultured and transfected with plasmid pCH1,pCH2(containing 6 kb and 11 kb human heart chymase genes,respectively) and pBS KS respectively according to the modification of the calcium phosphate precipitation method.After total RNA was isolated,RNA Dot blotting was carried out with 0.8 kb human heart chymase cDNA as the probe.The results showed that both human heart chymase genes(6 kb and 11 kb)expressed only in cultured nonmyocardial cells.The results indicated that the expression of human heart chymase gene was highly cellular specific and provided for further researches in the function, in vivo ,of chymase gene. |
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| Keywords: | Chymase gene Gene expression Myocardial cells Nonmyocardial cells |
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