| 葡萄糖和胰岛素对血管内皮细胞中microRNA-21的表达影响 |
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| 引用本文： | 刘显庆,姜德谦,罗玉梅,彭振宇,懂莉妮,嵇利亚,王镇河.葡萄糖和胰岛素对血管内皮细胞中microRNA-21的表达影响[J].现代生物医学进展,2010,10(6). |
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| 作者姓名： | 刘显庆 姜德谦 罗玉梅 彭振宇 懂莉妮 嵇利亚 王镇河 |
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| 作者单位： | 1. 中南大学湘雅二医院心血管内科,湖南,长沙,410011
2. 龙岗区人民医院心血管内科,广东,深圳,518172 |
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| 基金项目： | 深圳市重点资助项目(200643)资助 |
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| 摘 要： | 目的:研究miRNA-21和程序性细胞死亡因子4(PDCD4)在不同浓度葡萄糖或胰岛素培养血管内皮细胞中的表达情况,探讨miRNA-21对内皮细胞凋亡功能的影响。方法:用不同浓度D-葡萄糖(5.5、11、22及33mM)和胰岛素(0、1、10、50、100及1000nM)分别干预人脐静脉内皮细胞(HUVECs),2天后收集细胞用实时荧光定量RT-PCR方法检测细胞中miRNA-21及PDCD4的mRNA表达情况,用蛋白印迹方法检测PDCD4在细胞中的表达变化。结果:与5.5mM葡萄糖对照组相比,高葡萄糖浓度组(11、22及33mM)中HUVECs的miRNA-21随着葡萄糖浓度的增加表达明显降低(p<0.01);PDCD4的蛋白表达增加(p<0.05),但PDCD4的mRNA表达无差异(p>0.05)。与0nM胰岛素组相比,10、50、100、1000nM胰岛素组细胞中miRNA-21表达下降(p<0.05),PDCD4的蛋白表达增加(p<0.01),1000nM组miRNA-21表达降低程度和PDCD4的蛋白表达增加程度最为明显(p<0.05),但不同浓度胰岛素对PDCD4的mRNA表达无差异(p>0.05...
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| 关 键 词： | microRNA-21 程序性细胞死亡因子4 凋亡 血管内皮细胞 葡萄糖 胰岛素 |
The Influence of MicroRNA-21 Expression in Vascular Endothelial Cells Treated with High Glucose and Insulin |
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| LIU Xian-qing,JIANG De-qian,LUO Yu-mei,Peng Zhen-yu,DONG Li-ni,JI Li-ya,WANG Zhen-he.The Influence of MicroRNA-21 Expression in Vascular Endothelial Cells Treated with High Glucose and Insulin[J].Progress in Modern Biomedicine,2010,10(6). |
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| Authors: | LIU Xian-qing JIANG De-qian LUO Yu-mei Peng Zhen-yu DONG Li-ni JI Li-ya WANG Zhen-he |
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| Institution: | LIU Xian-qing1,JIANG De-qian1,LUO Yu-mei2,Peng Zhen-yu1,DONG Li-ni1,JI Li-ya1,WANG Zhen-he1 (1 Department of Cardiology,The Second Affiliated Hospital of Central South University,Hunan 410011,2 Department of Cardiology,The People Hospital of Longgang,Shenzhen Guangdong 518172,China) |
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| Abstract: | Objective To explore the expression and function of miRNA-21 and programmed cell death 4 (PDCD4) in Human umbilical vein endothelial cells (HUVECs) treated with high glucose or insulin.Methods: HUVECs were treated with D-Glucose in different concentrations (5.5mM,11 mM,22 mM and 33 mM) or insulin (0nM,1nM,10nM,50nM,100nM,1000nM) respectively for 2 days,and then cells were collected to detect miRNA-21 and PDCD4 mRNA expression levels with the real-time fluorescence quota RT-PCR method.The expression of prote... |
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| Keywords: | MicroRNA-21 Programmed cell death 4 Apoptosis Vascular endothelial cells High glucose High insulin |
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