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Antibodies to pollen exines |
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| Authors: | D. Southworth M. B. Singh T. Hough I. J. Smart P. Taylor R. B. Knox |
| Affiliation: | (1) Department of Biology, Southern Oregon State College, 97520 Ashland, OR, USA;(2) Plant Cell Biology Research Centre, Department of Botany, University of Melbourne, Melbourne, Australia;(3) Department of Agriculture and Rural Affairs, Veterinary Research Institute, 3052 Parkville, Vic, Australia |
| Abstract: | A polyclonal antiserum and monoclonal antibodies have been prepared to purified pollen exines of Calocedrus decurrens Florin. The location of the antigen is in the exine, as shown by light-and electron-microscopic immunocytochemistry. The greatest reduction in antibody binding follows treatment of the exine with chemicals known to alter sporopollenin. These results provide evidence that sporopollenin is antigenic. Exines of ten species of gymnosperms and angiosperms also bound the polyclonal antiserum, indicating similarity of sporopollenin structure. |
| Keywords: | Calocedrus Exine Mono-and polyclonal antibodies Pollen Sporopollenin |
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