Identification of embryonal antigens of maize: Globulins as primary reserve proteins of the embryo |
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Authors: | E E Khavkin S I Misharin Ye Yu Markov A A Peshkova |
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Institution: | (1) Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Academy of Sciences of USSR, P.O. Box 1243, 664033 Irkutsk 33, USSR |
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Abstract: | Normal and -irradiated caryopses of Zea mays L. were germinated, and the degradation of embryonal antigens (EA) was followed in the endosperms, scutella and embryonic axes of the seedlings, using double immunodiffusion, immunoelectrophoresis and quantitative immunoprecipitation. The predominant transient EA were presumed to be storage proteins related to the reserve globulins of dicotyledonous seeds. Therefore globulins were isolated from maize scutella, purified by (NH4)2SO4 fractionation and isoelectric precipitation, and the molecular weights of the polypeptide units were estimated by discontinuous sodium-dodecyl-sulphate slab electrophoresis. The globulins were found to be identical with the predominant EA and amounted to about 40% of the protein nitrogen in the embryos of mature, non-germinated caryopses. The presumed reserve function of the globulins and the characteristic time course of their degradation in embryonic axes and scutella of maize seedlings are discussed in relation to the two-step pattern of mobilization of nitrogen reserves in germinating cereal caryopses.Abbreviations AG, AR and AS
antisera to globulins and to root and scutellum proteins, respectively
- CA
common antigens
- DAP
days after pollination
- EA
embryonal antigens
- ME
2-mercaptoethanol
- SDS-PAGE
discontinuous sodium-dodecylsulphate slab polyacrylamide-gel electrophoresis
- TCA
trichloroacetic acid |
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Keywords: | Antigen spectra Embryo (proteins) -Plantlets" target="_blank">gif" alt="gamma" align="MIDDLE" BORDER="0">-Plantlets Germination (seeds) Globulins Proteins (storage) Seedling growth Storage proteins Zea |
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