Neuroprotective effect of small heat shock protein,Hsp27, after acute and chronic alcohol administration |
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Authors: | Melinda Erzsebet Toth Szilvia Gonda Laszlo Vigh Miklos Santha |
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Institution: | (1) Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701 Szeged, Hungary; |
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Abstract: | Alcohol induces degeneration of neurons and inhibits neurogenesis in the brain. Small heat shock proteins are able to protect
neurons in cerebral ischemia and oxidative stress. In this study, we investigated the neuroprotective effect of small heat
shock protein, Hsp27, after acute and chronic ethanol administrations using transgenic mice overexpressing the human Hsp27
protein. Transgenic mice and wild-type littermates were injected with 2 g/kg ethanol intraperitoneally, and then motor coordination
and muscle strength were analyzed using different behavioral tests, such as footprint analysis, balance beam, and inverted
screen tests. Ethanol-injected transgenic mice showed similar footprints to control saline-injected mice, did not fall of
the beam, and were able to climb to the top of the inverted screen, while wild-type mice showed ataxia and incoordination
after ethanol injection. The effect of Hsp27 on chronic ethanol consumption was also investigated. Drinking water of mice
was replaced by a 20% ethanol solution for 5 weeks, and then brain sections were stained with Fluoro Jade C staining. We found
significantly lesser amount of degenerating neurons in the brain of ethanol-drinking transgenic mice compared to wild-type
mice. We conclude that Hsp27 can protect neurons against the acute and chronic toxic effects of ethanol. |
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Keywords: | Hsp27 Neuroprotection Ethanol Transgenic mice |
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