| 牛红细胞超氧化物歧化酶活性中心的紫外光谱研究 |
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| 引用本文： | 梁贤振,李连之,胡绪英,周永洽,申泮文. 牛红细胞超氧化物歧化酶活性中心的紫外光谱研究[J]. 中国生物化学与分子生物学报, 1998, 14(2): 175-180 |
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| 作者姓名： | 梁贤振 李连之 胡绪英 周永洽 申泮文 |
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| 作者单位： | 中国科学院上海药物研究所，南开大学化学系 |
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| 摘 要： | 应用差示分光光度法研究了牛红细胞Ｃｕ２Ｚｎ２ＳＯＤ的紫外光谱，归属和讨论了酶活性中心金属离子与配体间全部电荷转移谱带，给出了相应的配体轨道光学电负性，特别研究了涉及Ｚｎ２＋的电荷转移谱带．
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| 关 键 词： | 超氧化物歧化酶 电荷转移光谱 紫外光谱 光学电负性 |
| 收稿时间： | 1998-04-20 |
The Study on the UV Spectra of the Active Center of Superoxide Dismutase of Bovine Erythrocytes |
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| [KHD〗Liang Xian Zhen. The Study on the UV Spectra of the Active Center of Superoxide Dismutase of Bovine Erythrocytes[J]. Chinese Journal of Biochemistry and Molecular Biology, 1998, 14(2): 175-180 |
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| Authors: | [KHD〗Liang Xian Zhen |
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| Affiliation: | (Shanghai Institute of Materia Medica,Chinese Academy of Science,Shanghai 200032) Li Lian Zhi Hu Xu Ying Zhou Yong Qia Shen Pan Wen (The Department of Chemistry,Nankai University,Tianjin 300071 |
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| Abstract: | The differential spectroscopy is used to study the UV spectra of Cu 2Zn 2SOD of bovine erythrocytes .Eight bands of the transfer spectra between ligand and metal charge(LMCT) of the enzyme active center are found.These LMCT absorptions originate in the structure of Cu(Π) imidazole and Cu(Π) imidazolate.The absorptions of σ(ImH)→,3π 2(ImH)→,π 1(ImH)→,(σ N+σ N)(Im -)→,(σ N-σ N)(Im -)→,π(Im -)→Cu 2+ occur at 238,253,259,265,330,235,350 and 282 nm,correspectively.Besides,their optical electronegativities are also given in correspond.Particularly,the spectra concerning Zn 2+ ion in this enzyme is studied,and two bands of 234 and 298nm are obesrved which originate probably from the LMCT absorption of Zn 2+ and its ligands. |
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| Keywords: | Superoxide dismutase Transfer spectra between ligand and metal charge UV spectra Electronegativity |
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