| 棉蚜体内感染沃尔巴克氏体（Wolbachia）的分子检测 |
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| 引用本文： | 国伟,沈佐锐.棉蚜体内感染沃尔巴克氏体（Wolbachia）的分子检测[J].微生物学杂志,2004,24(2):1-3. |
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| 作者姓名： | 国伟 沈佐锐 |
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| 作者单位： | 中国农业大学,农学与生物技术学院,北京,100094 |
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| 基金项目： | 瑞典国际基金IFS资助项目 (C/3 2 5 7-1)，国家 973重点项目“农作物重大病虫害成灾机理及调控基础的研究”(G2 0 0 0 0 162 10 -10 )资助 |
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| 摘 要： | Wolbachia是存在于节肢动物体内的一类呈母系遗传的细胞内共生细菌 ,这类细菌可以通过卵的细胞质传播并参与调控寄主的生殖活动。通过对Wolbachia外膜蛋白质的wsp基因进行特异性扩增 ,证实了寄生于不同植物的棉蚜 (AphisgossypiiGlover)体内均有感染 ,说明Wolbachia可能广泛存在于棉蚜体内 ,扩增出的Wolbachia目的片段为 5 90bp左右。通过对棉蚜体内感染的Wolbachia的wsp基因序列进行分子检测 ,为进一步研究棉蚜的孤雌生殖与Wolbachia的相关关系等奠定基础。
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| 关 键 词： | 沃尔巴克氏体(Wolbachia) wsp基因(沃氏体的外膜蛋白基因) 棉蚜 |
| 文章编号： | 1005-7021(2004)02-0001-03 |
| 修稿时间： | 2003年9月5日 |
Molecular Identification of wsp Gene of Wolbachia Infected in Aphis gossypii Glover |
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| GUO Wei,SHEN Zuorui.Molecular Identification of wsp Gene of Wolbachia Infected in Aphis gossypii Glover[J].Journal of Microbiology,2004,24(2):1-3. |
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| Authors: | GUO Wei SHEN Zuorui |
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| Abstract: | Wolbachia, found in reproductive tissues of arthropods, are obligatory, maternally inherited intracellular bacteria. These bacteria are transmitted through the cytoplasm of eggs and have evolved various mechanisms for manipulating reproduction of their hosts. Based on the amplification of part of wsp gene encoding the outer membrane protein of Wolbachia, the presence of Wolbachia has been successfully detected in Aphis gossypii Glover. It is shown that perhaps Wolbachia are bacteria widespread in cotton aphids. After molecular identification of wsp gene, some factors contributing to this uncongruence are discussed. |
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| Keywords: | Wolbachia wsp gene Aphis gossypii Glover |
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