Neurogenic-committed human pre-adipocytes express CYP1A isoforms |
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| Authors: | Chiara Scanarotti Anna Maria Bassi Mariafrancesca Catalano Chiara Guida Rosella Coradeghini Carla Falugi Mariagrazia Aluigi Pierluigi Santi Edoardo Raposio |
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| Institution: | 1. Cell Culture Lab, DIMES, University of Genoa, Via L.B. Alberti 2, 16132 Genoa, Italy;2. Tissue Engineering Lab, Plastic Surgery Division, DICMI, University of Genoa, Via De Toni 14, 16132 Genoa, Italy;3. Nanobiosciences Unit, JRC - European Commission Institute for Health and Consumer Protection, Via E. Fermi 1, 21020 Ispra (VA), Italy;4. Biology Department, DIBIO, University of Genoa, Viale Benedetto XV 5, 16132 Genoa, Italy;5. National Cancer Institute, IST, University of Genoa, L.go R. Benzi 10, 16132 Genoa, Italy;1. Jules Verne University of Picardy, Amiens, France;2. Paris Descartes University, Paris, France;3. Pierre and Marie Curie University, Paris, France;4. Department of Maxillofacial Surgery, Amiens University Medical Center, Amiens, France;5. Department of Maxillofacial and Plastic Surgery, Necker Children''s Hospital, Paris, France;6. Facing Faces Institute, Amiens University Medical Center, Amiens, France;1. Resident, Department of Oral & Maxillofacial Surgery, King George Medical University, Lucknow, India;2. Professor, Department of Oral & Maxillofacial Surgery, King George Medical University, Lucknow, India;1. Department of Radiology, Queen Alexandra Hospital, Portsmouth PO6 3LY, UK;2. Department of ENT, Queen Alexandra Hospital, Portsmouth PO6 3LY, UK;3. Department of Oral and Maxillofacial Surgery, Queen Alexandra Hospital, Portsmouth PO6 3LY, UK;1. Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama 790-8577, Japan;2. Department of Life and Nanopharmaceutical Science, Kyung Hee University, Seoul 130-701, Republic of Korea;3. Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea;4. Graduate School of Science and Engineering, Ehime University, Matsuyama 790-8577, Japan;1. Neonatology Service of the “Dr. José Eleuterio González” University Hospital, Autonomous University of Nuevo Leon, Mexico;2. Genetics Service of the “Dr. José Eleuterio González” University Hospital, Autonomous University of Nuevo Leon, Mexico;3. Pediatrics Department of the “Dr. José Eleuterio González” University Hospital, Autonomous University of Nuevo Leon, Mexico |
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| Abstract: | Stem cell models offer an opportunity both for therapeutic use and for the assessment of alternative in vitro models. Human lipoaspirate is a source of adult stem cells (pre-adipocytes), which are able to differentiate into various phenotypes, such as neurogenic lineage. Here, we analyse the suitability of these in vitro models in screening exogenous compounds, such as environmental pollutants, that may affect adipose cells and neurogenic development. To evaluate neurogenic differentiation, we analysed expression of cholinergic system and acetylcholinesterase immunoreactivity. Heterocyclic derivatives of polycyclic aromatic hydrocarbons (PAHs) are often significant components of environmental contaminants. As they contain inducers of cytochrome P450 1A1 (CYP1A1), we explored the activity of CYP1A1-related enzymes, i.e. 7-ethoxycoumarin- and 7-ethoxyresorufin-O-deethylase (ECOD and EROD) in both cell systems in basal conditions and after exposure to non-cytotoxic doses of β-naphthoflavone (BNF), a well-known PAH-type inducer. Both cell models showed basal and inducible levels of ECOD. Analysis of CYP1A1 protein expression and EROD-related enzyme activity confirmed the inducibility of the CYP1A1 isoform by BNF. These results demonstrate that mesenchymal adult stem cells can constitute innovative models. We therefore propose the use of pre-adipocytes and their neurogenic derivates to evaluate the cytotoxic/biological effects of unintended exposure to contaminants. |
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