The -bearing RI segment of the chromosome |
| |
Authors: | Evelyn A Devine Mary C Moran Peter J Jederlinic Anthony J Mazaitis Henry J Vogel |
| |
Institution: | Department of Pathology, College of Physicians and Surgeons Columbia University, New York, New York 10032 USA |
| |
Abstract: | The transducing phage λd, carrying a portion of the chromosome including , is derived from the heat-inducible, lysis-defective strain λy199, which has the and deletions. Cleavage of λy199 DNA by RI endonuclease, followed by agarose slab gel electrophoresis, results in bands corresponding to the known C, D, E, and F segments of λ, and a segment A′ (A plus B minus minus , the cleavage site between A and B being eliminated). Cleavage of λd DNA by RI yields the expected D, E, and F segments of λ and four other segments, termed 14-1 through 14-4, whose length is 17.5, 6.2, 3.0, and 2.0 kilobases, respectively, as determined by electron microscopy and corroborated by electrophoretic mobility. Heteroduplex analysis shows that the cluster is on the 14-1 segment. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|