| ‘巴斗杏’组培快繁体系建立与耐盐植株筛选 |
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| 引用本文： | 杜雪玲,余如刚,宋运贤,张振霞.‘巴斗杏’组培快繁体系建立与耐盐植株筛选[J].亚热带植物科学,2011,40(2):34-38. |
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| 作者姓名： | 杜雪玲 余如刚 宋运贤 张振霞 |
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| 作者单位： | 1. 淮北师范大学生命科学学院资源植物学安徽省重点实验室,安徽淮北,235000
2. 韩山师范学院生物系,广东潮州,521041 |
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| 基金项目： | 安徽省高校自然科学研究项目 |
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| 摘 要： | 以淮北黄里‘巴斗杏’茎段为外植体,MS为基本培养基,通过茎段诱导植株再生及进行耐盐筛选。结果表明:采用4、5月‘巴斗杏’茎段用0.1%升汞灭菌8 min较适宜;在含有1.0 mg/L 6-BA和0.1 mg/L IBA的MS培养基上茎段增殖较快,生长旺盛;较理想的生根培养基为MS+NAA 0.1 mg/L+IBA 0.2 mg/L,生根率达46.3%;‘巴斗杏’组培苗进行耐盐筛选的适宜盐浓度为0.4%～0.8%,筛选植株与对照相比差异显著。
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| 关 键 词： | ‘巴斗杏’ 腋芽 增殖 生根 耐盐筛选 |
Establishment of Tissue Culture and Rapid Propagation System of Armeniaca vulgaris 'Badou'and Selection of Salt-tolerant Plant |
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| DU Xue-ling,YU Ru-gang,SONG Yun-xian,ZHANG Zhen-xia.Establishment of Tissue Culture and Rapid Propagation System of Armeniaca vulgaris 'Badou'and Selection of Salt-tolerant Plant[J].Subtropical Plant Science,2011,40(2):34-38. |
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| Authors: | DU Xue-ling YU Ru-gang SONG Yun-xian ZHANG Zhen-xia |
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| Institution: | DU Xue-ling1,YU Ru-gang1,SONG Yun-xian1,ZHANG Zhen-xia2(1.Anhui Key Laboratory of Plant Resources and Biology,College of Life Science,Huaibei Normal University,Huaibei 235000,Anhui China,2.Department of Biology,Hanshan Normal University,Chaozhou 521041,Guangdong China) |
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| Abstract: | The stem of Armeniaca vulgaris 'Badou' was used as explants and MS as basic substrate to induce plant regeneration and select salt-tolerant plant.The results showed that the stem of 'Badou' in April and May sterilized by 0.1% mercury for 8min was suitable,the stem in MS culture medium with 1.0mg/L 6-BA and 0.1 mg/L IBA proliferated quickly and grew rushingly,the perfect rooting medium was MS+0.1 mg/L NAA + 0.2 mg/L IBA,with its rooting rate of 46.3%,and the optimum salt concentration in selection of salt-to... |
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| Keywords: | Armeniaca vulgaris 'Badou' axillary buds proliferation rooting selection of salt-tolerant |
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