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Detection, cultivation and maintenance of Gallionella in laboratory microcosms
Authors:J Verran  JFD Stott  SL Quarmby  M Bedwell
Institution:Department of Biological Sciences, Manchester Metropolitan University, Manchester, UK;Corrosion and Protection Centre Industrial Services (CAPCIS), UMIST, Manchester, UK
Abstract:A simple circulating system was developed whereby growth medium or stream water was circulated through vessels containing putative Gallionella specimens. An iron source was provided, separate from the growth vessel. Removal of the iron source resulted in a rapid depletion of iron II ions indicative of Gallionella activity. Presence and viability of Gallionella were confirmed microscopically and by culture. The system is suitable for corrosion studies under conditions of controlled water chemistry, as well as for studies involving removal of metal ions from waste waters and aquifers.
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