Multicolour FISH detects frequent chromosomal mosaicism and chaotic division in normal preimplantation embryos from fertile patients |
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Authors: | J D A Delhanty Joyce C Harper Asangla Ao Alan H Handyside Robert M L Winston |
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Institution: | (1) Human Genetics Group, Galton Laboratory, Wolfson House, University College London, 4 Stephenson Way, London, NW1 2HE, UK Tel.: +44-171-380-7409; Fax: +44-171-380-7429, GB;(2) Institute of Obstetrics & Gynaecology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London, W12 0NN, UK, GB |
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Abstract: | We have used multicolour fluorescent in situ hybridisation (FISH) with DNA probes for chromosomes X, Y and 1 to analyse spare
untransferred cleavage-stage embryos after preimplantation diagnosis to avoid X-linked disease. In total, 93 morphologically
normal embryos were available from seven patients (six of proven fertility) who had undergone fourteen in vitro fertilisation
(IVF) cycles. The chromosome patterns observed were classified into four groups; normal, abnormal (non-mosaic), mosaic and
chaotic (uncontrolled division). Approximately half of the embryos were normal for the chromosomes tested. Two embryos only
were aneuploid (non-mosaic) throughout but, after excluding those showing chaotic division, 30% were considered to be chromosomal
mosaics. Of these, a minority had arisen because of mitotic non-disjunction or chromosome loss or gain, whereas the majority
were ploidy mosaics, with haploidy being the most common. The occurrence of chaotically dividing embryos was strongly patient-related,
i.e. some patients had ‘chaotic’ embryos in repeated cycles, whereas other patients were completely free of this type of anomaly.
‘Chaotic’ embryos are unlikely to progress beyond implantation. These findings have important implications both for routine
IVF and preimplantation genetic diagnosis.
Received: 18 October 1996 / Revised: 23 January 1997 |
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