Callus formation from protoplasts of a sugarbeet cell suspension culture |
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Authors: | László Szabados Carina Gaggero |
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Institution: | (1) Sugarbeet Research Institute, H-9463 Sopronhorpács, Hungary;(2) Present address: CIAT, Apdo. aéro 6713, Cali, Colombia |
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Abstract: | Protoplasts of sugarbeet (Beta vulgaris L.) were isolated from cell suspension cultures and cultured in modified PGo medium. Conditions required for the efficient division of the protoplasts were investigated.The optimal combination of phytohormones was found to be 1 mg/l NAA, 0.2 mg/l 2,4-D, 0.5 mg/l zeatin. Protoplast division was also considerably stimulated by the addition of 250 mg/l casein hydrolysate, 200 mg/l yeast extract, and 20% v/v conditioned culture medium to the protoplast culture medium. The highest division rate (up to 35% of the protoplasts) was achieved at a density of 4×104- 1×105 protoplasts/ml. From the colonies callus and suspension cultures were readily obtained.Abbreviations BAP
6-Benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
Indole-3-acetic acid
- Kin
6-Furfurylaminopurine (kinetin)
- NAA
-Naphtalenacetic acid
- Zea
Zeatin |
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