衣藻生物制氢的研究进展

综述了利用衣藻生产氢气作为再生能源的研究进展。分别介绍了衣藻产氢的代谢机理、培养条件、衣藻氢化酶的特性以及利用分子生物学手段、生物信息学手段和生物工程技术提高衣藻生物制氢效率的方法，包括氢化酶的氧耐受性的改造、外源氢化酶基因的表达、影响衣藻产氢的关键基因的筛选、利用缺硫培养基和固定化培养方法提高氢气产量等。最后，还对利用衣藻生物制氢的可行性和经济性进行了分析，对其发展方向提出自己的看法。

Advances of Bio-hydrogen Production in Chlamydomonas reinhardtii

This article has a review on the research advances of hydrogen production in Chlamydomonas reinhardtii as a renewable energy source. The mechanism of hydrogen metabolism, culture conditions, characteristics of hydrogenases and approaches to improve hydrogen productivity in C. reinhardtii using molecular biological methods, bioinformatics and bioengineering technology were addressed. The approaches includes the modification of hydrogenase's tolerance to O2, heterologous expression of hydrogenase genes, screening of enzymes essentially for H2 production, using S-deprived medium, cell immobilization or 2-stage culture method to improve H2 production. The possibility, economics and trends of bio-hydrogen production using C. reinhardtii are also analyzed.