Bacteriorhodopsin,boundary lipid and protein conformers. A spin label study |
| |
| Authors: | Winfried Hoffmann Anthony D. Clark Margaret Turner Sidney Wyard Dennis Chapman |
| |
| Affiliation: | 1. Royal Free Hospital School of Medicine, Department of Biochemistry and Chemistry, 8 Hunter Street, London WC1N 1BP U.K.;2. Guy''s Hospital Medical School, Department of Physics, London Bridge, London SE1 9RT U.K. |
| |
| Abstract: | ![]()
A spin label study, as a function of temperature, has been made with the bacteriorhodopsin membrane using a stearic acid spin label. The ESR spectra show a strong variation with temperature and the presence of isosbestic points. The spectra are interpreted as indicating the presence of a two-component system with an activation energy (approx. 14 kcal/mol) corresponding to a protein conformational change. This activation energy is similar to that deduced from recent flash photolysis studies.It is concluded that the spin label is sensitive to the temperature-dependent protein conformational change in this membrane system. |
| |
| Keywords: | Bacteriorhodopsin Boundary lipid Protein conformer Spin label Temperature effect Isosbestic point |
| 本文献已被 ScienceDirect 等数据库收录！ |
