The binding of cytochrome to plasma membranes of rat liver. Its implication for membrane specificity and biogenesis |
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Authors: | José Remacle |
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Institution: | 1. Department of Biology, University of California at San Diego, La Jolla, CA 92037 U.S.A.;2. Laboratoire de Biochimie Cellulaire, Facultés Universitaires, B-5000 Namur Belgium |
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Abstract: | The in vitro incorporation of cytochrome into purified plasma membranes was investigated by biochemical and immunological methods. Plasma membrane preparations incorporated three times less cytochrome than did microsomal preparations; 60% of this cytochrome could not be reduced by the NADH-cytochrome reductase and was considered as being bound to the plasma membrane. The morphological observations made after the immunochemical labeling of cytochrome clearly showed a good but asymmetrical distribution of the ferritin labeling: only the inner face of the plasma membrane incorporated cytochrome . These results are discussed with respect to theories which concern the subcellular membrane relationships in the cell. |
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Keywords: | Membrane specificity Membrane synthesis (Hepatocyte plasma membrane) |
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