Sphingosine and FTY720 modulate pacemaking activity in interstitial cells of Cajal from mouse small intestine |
| |
Authors: | Joo Hyun Nam Woo Kyung Kim Byung Joo Kim |
| |
Institution: | 1. Department of Physiology, Dongguk University College of Medicine, Kyungju, 780-714, Korea 2. Department of Internal Medicine, Dongguk University College of Medicine, Goyang, 410-773, Korea 3. Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, 626-870, Korea
|
| |
Abstract: | Interstitial cells of Cajal (ICCs) are the pacemakers of the gastrointestinal tract, and transient receptor potential melastatin type 7 (TRPM7) and Ca2+ activated Cl− channels (ANO1) are candidate the generators of pacemaker potentials in ICCs. The effects of D-erythro-sphingosine (SPH) and structural analogues of SPH, that is, N,N-dimethyl-Derythro-sphingosine (N,N-DMS), FTY720, and FTY720-P on the pacemaking activities of ICCs were examined using the whole cell patch clamp technique. SPH, N,N-DMS, and FTY720 decreased the amplitudes of pacemaker potentials in ICC clusters, but resting membrane potentials displayed little change. Also, perfusing SPH, N,N-DMS, or FTY720 in the bath reduced both inward and outward TRPM7-like currents in single ICCs, and inhibited ANO1 currents. The another structural analogue of SPH, FTY720-P was ineffective at the pacemaker potentials in ICC clusters and the TRPM7-like currents in single ICCs. Furthermore, FTY720- P had no effect on ANO1. These results suggest that SPH, N,N-DMS, and FTY720 modulate the pacemaker activities of ICCs, and that TRPM7 and ANO1 channels affect intestinal motility. |
| |
Keywords: | ANO1 interstitial cells of Cajal pacemaker potentials Sphingosine TRPM7 |
本文献已被 SpringerLink 等数据库收录! |
|