Genetic diversity and population structure analysis of Qinghai-Tibetan plateau schizothoracine fish (Gymnocypris dobula) based on mtDNA D-loop sequences |
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| Institution: | 1. Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Ministry of Education, College of Marine Sciences, Shanghai Ocean University, 999 Huchenghuan Road, Lingang New City, Shanghai, 201306, PR China;2. National Distant-water Fisheries Engineering Research Center, Shanghai Ocean University, Shanghai, 201306, PR China;3. Collaborative Innovation Center for Distant-water Fisheries, Shanghai, 201306, PR China;1. Laboratório de Diagnósticos por DNA, Universidade do Estado do Rio de Janeiro, Brazil;2. Laboratório de Ecologia de Peixes, Universidade do Estado do Rio de Janeiro, Brazil;3. Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Portugal;4. Instituto de Patologia Molecular e Imunologia (IPATIMUP)/Instituto de Investigação e Inovação em Saúde, Universidade do Porto/Faculdade de Ciências, UP, Portugal;1. College of Life Science, Henan Normal University, Xinxiang 453007, PR China;2. College of Fisheries, Henan Normal University, Xinxiang 453007, PR China;1. Faculty of Fisheries, Muğla Sıtkı Koçman University, Muğla, 48000, Turkey;2. Department of Fisheries and Aquaculture, Faculty of Agriculture, Ankara University, Ankara, 06100, Turkey;3. Department of Medicinal and Aromatic Plants, Köyceğiz Vocational School, Muğla Sıtkı Koçman University, Muğla, 48800, Turkey;4. Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, Muğla, 48000, Turkey;5. Department of Biology, Faculty of Science, Eskişehir Osmangazi University, Eskişehir, 26040, Turkey;1. Department of Biology, Universiti Brunei Darussalam, Bandar Seri Begawan, Gadong, Negara, Brunei Darussalam;2. Institute of Ocean and Earth Science, University of Malaya, Kuala Lumpur, Malaysia;3. Department of Biology, University of Padova, via G. Colombo 3, 35121 Padova, Italy;4. Department of Comparative Biomedicine and Food Science, University of Padova, viale dell’Università 16, Agripolis 35020, Legnaro (PD), Italy;5. Naturhistorisches Museum der Burgergemeinde Bern, Bernastrasse 15, 3005 Bern, Switzerland |
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| Abstract: | Gymnocypris dobula is a commercially important fishery species and mostly distributed in the freshwater of Tibetan plateau. In this study, genetic diversity and intraspecific population differentiation were examined by using mitochondrial DNA D-loop sequences in 97 individuals sampled from three localities (Pali, Lasa and Yanghu) in the Tibetan plateau. Two hundred and fourteen polymorphic sites and 50 haplotypes were defined among the three localities. Genetic diversity analysis showed that the highest genetic diversity level was found in Pali population. Phylogenetic relationships analysis results indicated that closer phylogenetic relationships were found between the Yanghu and Lasa populations. Genetic population differentiation analysis indicated that the majority of variation (84.91%) was attributed to variations among populations and the largest differentiation was found between Pali and Lasa localities (Fst = 0.874, Nm = 0.036). Moreover, the historical demographic events were assessed by implementing the mismatch distribution analysis, Fajima's D test and Fu's Fs test. The results indicated that the Pali population had undergone a demographic expansion, possibly within the last 0.163 MYA (Million Years Ago). Our study firstly identified the population genetic structure of the G. dobula, which could be helpful for artificial breeding, fishery stock identification and resource conservation for this species. |
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| Keywords: | D-loop Genetic diversity Phylogenetic relationships Population structure |
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