On‐Line Activity Screening for Radical Scavengers from Baccharis chilco |
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| Authors: | Juan C Argoti Pablo J Linares‐Palomino Sofía Salido Bernardo Ramírez Braulio Insuasty Joaquín Altarejos |
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| Institution: | 1. Grupo de Investigación de Compuestos Heterocíclicos, Departamento de Química, Universidad del Valle, A.A. 25360 Cali, Valle del Cauca, Colombia, (phone: +57‐2‐3393248;2. fax: +57‐2‐3393248);3. Grupo de Investigación Química de Compuestos Bioactivos, Departamento de Química, Facultad de Ciencias Naturales, Exactas y de la Educación, Universidad del Cauca, Cauca, Colombia;4. Departamento de Química Inorgánica y Orgánica, Facultad de Ciencias Experimentales, Universidad de Jaén, ES‐23071 Jaén (phone: +34‐953‐212743;5. fax: +34‐953‐211876);6. Departamento de Biología, Facultad de Ciencias Naturales, Exactas y de la Educación, Universidad del Cauca, Cauca, Colombia |
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| Abstract: | Baccharis plants have been used since ancient times in American traditional medicine. Baccharis chilco is a perennial shrub of temperate regions of South America that grows well in rainfall forests of Colombia. Neither chemical composition nor biological studies of this plant have ever been reported. Two caffeoylquinic acid (CQA) derivatives, 5‐O‐(E)‐caffeoyl]quinic acid ( 1 ) and 3,5‐di‐O‐(E)‐caffeoyl]quinic acid ( 3 ), and rosmarinic acid ( 2 ) have been isolated from B. chilco growing wild in Colombia, using the on‐line HPLC‐DAD‐DPPH radical‐scavenging detection technique as guidance. In the course of the purification work, L ‐chiro‐inositol ( 4 ) was also isolated. Structures of the four isolated compounds were determined by spectroscopic methods. Antioxidants 2 and 3 exhibited high antiradical activities evaluated by the 2,2‐diphenyl‐1‐picrylhydrazyl radical (DPPH.) assay, although somewhat lower than that of the reference compound ascorbic acid. The on‐line HPLC‐DAD‐DPPH technique allowed a rapid pinpointing of antioxidants in the studied EtOH extract, and the facile guided isolation of the target molecules. |
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| Keywords: | Baccharis chilco Antioxidant activity Caffeoylquinic acid derivatives Rosmarinic acid chiro‐Inositol Radical‐scavenging activity |
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