Effect of a trypsin inhibitor from Dimorphandra mollis seeds on the development of Callosobruchus maculatus |
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Institution: | 1. Mirimus Inc, 760 Parkside Ave. Suite 206, Brooklyn, NY 11226, USA;2. Department of Pathology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11226, USA;3. Department of Cell Biology, SUNY Downstate Health Sciences University, 450 Clarkson Ave., Brooklyn, NY 11226, USA;4. MedStar Sports Medicine Research Center, MedStar Health Research Institute, 2900 S Hanover St., Baltimore, MD 21225, USA;5. Department of Rehabilitation Medicine, Georgetown University Medical Center, 3800 Reservoir Rd NW, Washington, DC 20007, USA;6. Minnesota Timberwolves, 600 Hennepin Ave, Minneapolis, MN 55403, USA;7. Department of Epidemiology of Microbial Diseases, Yale School of Public Health, 60 College St, New Haven, CT 06510, USA;8. OptumLabs, UnitedHealth Group, 12700 Whitewater Dr, Minnetonka, MN 55343 USA |
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Abstract: | Bruchid larvae cause major losses in grain legume crops throughout the world. Some bruchid species, such as the cowpea weevil, are pests that damage stored seeds. Plants synthesize a variety of molecules, including proteinaceous proteinase inhibitors, to defend themselves against attack by insects. In this work, a trypsin inhibitor (DMTI-II) isolated from Dimorphandra mollis seeds was tested for anti-insect activity against Callosobruchus maculatus larvae. The inhibitor produced ca. 67% mortality to this bruchid when incorporated into an artificial diet at a level of 1%. The doses necessary to cause 50% mortality (LD50) and to reduce weight by 50% (ED50) for DMTI-II were ca. 0.50% and 0.60%, respectively. The action of DMTI-II on C. maculatus larvae may involve the inhibition of trypsin-like activity of larval midgut extracts, the absence of digestion by midgut preparations or with a mixture of pepsin and papain, and its association with a chitin column and chitinous structure in the midgut of this insect. |
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