| 人TNFAIP1基因克隆及其在常见细胞系中的表达 |
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| 引用本文： | 杨利平,周爱冬,李 红,张文峰,吴艳阳,张 健,韩 梅.人TNFAIP1基因克隆及其在常见细胞系中的表达[J].遗传,2006,28(8):918-922. |
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| 作者姓名： | 杨利平 周爱冬 李 红 张文峰 吴艳阳 张 健 韩 梅 |
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| 作者单位： | 湖南师范大学生命科学学院，蛋白质化学与发育生物学教育部重点实验室，长沙 410081 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，高等学校博士学科点专项科研项目，教育部长江学者奖励计划 |
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| 摘 要： | 人类TNFAIP1蛋白是第一个被鉴定受肿瘤坏死因子α诱导的蛋白,有实验证明TNFAIP1 蛋白可能参与DNA复制、合成、细胞凋亡以及人类的各种疾病等重要过程，但对其确切功能和作用机制研究不多。本文克隆人的TNFAIP1基因到GST原核表达载体，并在原核细胞进行表达纯化。利用实时定量PCR的方法检测其在几种常用的细胞系的表达情况，发现在COS7以及NIH3T3细胞系中高表达，而在HeLa、HepG2、SW480、PanC1、MCF7等癌细胞系中低表达。由此推测TNFAIP1可能在癌症的发生中起到一定的作用。
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| 关 键 词： | western blot 肿瘤坏死因子α 实时定量PCR TNFAIP1 |
| 文章编号： | 0253-9772(2006)08-0918-05 |
| 收稿时间： | 2005-12-31 |
| 修稿时间： | 2006-03-06 |
Cloning and expression profile in the cell lines of human TNFAIP1 |
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| YANG Li-Ping,ZHOU Ai-Dong,LI Hong,ZHANG Wen-Feng,WU Yan-Yang,ZHANG Jian,HAN Mei.Cloning and expression profile in the cell lines of human TNFAIP1[J].Hereditas,2006,28(8):918-922. |
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| Authors: | YANG Li-Ping ZHOU Ai-Dong LI Hong ZHANG Wen-Feng WU Yan-Yang ZHANG Jian HAN Mei |
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| Institution: | Key laboratory of Protein Chemistry and developmental Biology, State Education ministry of China, College of Life Science , Hunan Normal University, Changsha 410081,China |
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| Abstract: | TNFAIP1 was first identified as a tumor necrosis factor alpha(TNFalpha) and interleukin-6 (IL-6) inducible protein. Experiments on human and rat suggested the protein may play roles in the DNA repair, DNA synthesis, cell apoptosis and human diseases. But its real function and mechanism have not been reported. In this paper, the expression of TNFAIP1 were determined in a number of cell lines by Real-time PCR. We found that the expression levels of TNFAIP1 are high in COS7 and NIH3T3 cell lines but low in the cancer cell lines. These suggest that TNFAIP1 may be involved in the process of cancer. The over-expression of human TNFAIP1 can accelerate the apoptosis of HeLa cell. |
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| Keywords: | TNFAIP1 Western-blot |
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