膜荚黄芪SCAR标记的建立

用RAPD方法对膜荚黄芪和蒙古黄芪进行指纹图谱的研究。采用BSA法从120个10碱基随机引物筛选出7个在膜荚黄芪基因池和蒙古黄芪基因池中表现多态性的引物。单株检测表明，引物OPD14具有膜荚黄芪特异性，在检测的膜荚黄芪个体中均能各自扩增出一条300 bp左右的特异带，而在蒙古黄芪的单株中则未见，将该膜荚黄芪特异性片断命名为OPD14_-300。获得的RAPD标记OPD14_-300经克隆、测序、重新设计一对特异性引物转化成更稳定的SCAR标记；该SCAR标记只在膜荚黄芪个体中出现，达到了在分子水平上快速、准确地鉴定膜荚黄芪和蒙古黄芪的目的。

The Establishment of SCAR Marker Linked to Astragalus membranaceus

RAPD analysis was adopted to identify Astragalus membranaceus Bge. and Astragalus membranaceus var. mongholicus(Bge.)Hsiao. With the method of BSA, 120 decamer random primers were used to amplify the DNA pools from A. membranaceus and A. membranaceuss var. mongholicus, and seven polymorphic primers were screened. Those polymorphic primers were used to detect the individual samples, a band of 300 bp from primer OPD14 was specifically amplified in the tested A. membranaceus and were absent in the tested A. membranaceuss var. mongholicus. This indicted that the band had specific character of A. membranaceus and marked as OPC14_-300. SCAR marker was developed from the RAPD marker OPD14_-300 by cloning, sequencing and designing one pair of primers and the SCAR marker only presented in the individuals of A. membranaceus, thus, it can be used to identify A. membranaceus and A. membranaceuss var. mongholicus at molecular level quickly and accurately.